Aburegeba, Zina - Characterization of suppressors of fat-like cadherin CDH-4 in context of axon guidance during embryo...

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This thesis has been submitted to the Library for purposes of graduation, but needs to be audited for technical details related to publication in order to be approved for inclusion in the Library collection.
Term: 
Spring 2018
Degree: 
M.Sc.
Degree type: 
Thesis
Department: 
Biological Sciences Department
Faculty: 
Science
Senior supervisor: 
Harald Hutter
Thesis title: 
Characterization of suppressors of fat-like cadherin CDH-4 in context of axon guidance during embryonic development
Given Names: 
Zina
Surname: 
Aburegeba
Abstract: 
Developing the nervous system requires axons to grow and connect to specific targets, forming neuronal circuits, through axon navigation. One of the genes controlling axon navigation is the fat-like cadherin CDH-4, a member of the cadherin superfamily. To identify genes acting together with cdh-4, mutants suppressing the axon guidance defects of cdh-4 mutants were isolated previously. The goal of this thesis was to characterize and identify these suppressor genes. Five suppressors were studied in detail. They partially suppressed axon guidance and movement defects observed in the cdh-4 mutants. Three suppressor genes were identified: math-48, a functionally uncharacterized gene, prp-6 and prp-8, both encoding components of the spliceosome. A previous study documented changes in expression of several hundred genes when prp-6 or prp-8 are partially inactivated. This suggests that the suppression effect might be mediated by upregulation of one or more genes, compensating for the loss of CDH-4.
Keywords: 
cdh-4; C. elegans; Ventral nerve cord; Dorsal nerve cord; Suppressors; Axon Guidance; Nervous system development
Total pages: 
117